Part of the A14 will be closed, including tonight (November 20) and tomorrow, while a temporary bridge is taken down at Swavesey.
The bridge had been used to carry materials across the carriageway during construction of the road's £1.5 billion upgrade but is no longer needed.
The road will be closed between Godmanchester and Girton, eastbound tonight, westbound tomorrow and in both directions on Saturday. The closures take place between 9pm and 5am and diversions have been set up along the A1198 and A428.
Local traffic will still be able to travel eastbound to Fenstanton where the road will close and westbound vehicles will be able to travel to Swavesey.
Chris Griffin, Highways England project manager, said: "Using this temporary bridge has helped us carry out work to prepare the construction of the future Swavesey interchange with minimal impact on people's journeys and is just one of the ways in which we are keeping traffic moving while we deliver these vital improvements for the A14.
"The bridge will be taken down using temporary overnight closures on the 14, so I encourage drivers to plan ahead and find out what to do if they are affected."
The bridge has been used to carry 160,000 cubic metres of materials, enough to fill 65 Olympic swimming pools, from the south to the north of the A14 in two months. Without it, an extra 40,000 lorry movements on the A14 would have been needed.